Abstract: Dermatophytosis is a common infection involving hair, skin or nail/caused by related fungal species belonging to three genera, Microsporum, Epidermophyton and Trichophyton. This study was done to have a general idea about dermatophytosis status in Makkah region and their main causative agents. Clinical specimens were collected from 63 patients attending different dermatology clinics in the main public and specialized hospitals in Makkah city. The study was carried out in the period of February to November 2011. The collected specimens were processed by direct examination using KOH preparation and by culture on SDA. Dermatophytes species were identified based on cultures morphology and microscopy. In addition, the laboratory records of 103 dermatophytes suspected cases seen at King Khalid National Guard Hospital during the period between 2009 and 2011 were retrieved and analyzed. Trichophyton was the most common etiological agent in this study (13 out of 63 and 8 out of 103). The second most common species were belonging to the genus Microsporum.