Authors : Sattar Beig Mohammadi, Kian Pahlevan Afshari and Hamid Reza Seyedabadi
Abstract: Native goats of importance in the economy of rural households are also important as genetic reserves that account for the reserving genetic diversity in native goat breeds of Iran because of the little population size is necessary for breeding goals and increasing their production. The first step is determination of genetic diversity in existing populations. Among the genetic markers, mtDNA sequencing is one of the most useful and common methods employed for inferring phylogenetic relationship between close related species and population and conservation of species. The object this study was carried out for determination of the mitochondrial ND6 gene sequence in Mahabadi native goat in Iran. For this study blood samples were taken randomly from 30 goats. After extracting DNA, ND6 gene of mtDNA was amplified with specific primers using PCR and after purification was sequenced. The phylogenic tree was drawn with the consensus sequence of other similar sequences of different important livestock species obtained from GenBank. In the phylogenic tree, Mahabadi native goat was clustered with such as sheep, cattle, pig, horse, camel and swamp buffalo breeds. This is possible because of the conserved area is ND6 in goats.
Sattar Beig Mohammadi, Kian Pahlevan Afshari and Hamid Reza Seyedabadi, 2020. ondrial Diversity and Phylogenetic Structure of Native Iranian Goat Population Compared to Different Important Livestock Species. Research Journal of Animal Sciences, 14: 38-44.