Abstract: The purpose of the study was to determine alterations in the hematological, biochemical and coagulation parameters in calves with Lipopolysaccharide (LPS)-induced experimental endotoxemia. Endotoxemia was induced via intravenous administration of LPS (0.1 μg kg-1) in 50 mL of physiological 0.9% NaCl >30 min. The levels of serum Alanine aminotransferase (ALT), Gamma-glutamyl Transferase (GGT), Aspartate aminotransferase (AST), Blood Urea Nitrogen (BUN), Total Protein (TP), Lactate Dehydrogenase (LDH), Alkaline Phosphatase (ALP), total bilirubin, glucose, Creatinine (Cre), Activated Partial Thromboplastin Time (APTT), Prothrombin Time (PT) and fibrinogen were measured. Severe abnormality were observed in some of these parameters. Changes in hematological, biocehmical and coagulation findings were related to endotoxemia. The LPS infusion resulted in significant changes of haematological, biochemical and coagluation parametrers.