Abstract: Bio-fertilizers are low cost renewable source of plant nutrients which supplement chemical fertilizers. In this study, researchers determine the influence of exogenously applied two bio-fertilizers humic acid and nitroxin on morphological characteristics, fruit yield and quality of cucumber grown under direct soil conditions in a fall planting. The experiments were carried out in a factorial trial based on completely randomized block design with three replicates. The treatments were foliar application of humic acid at 0, 10, 20 and 30 mL-1 and nitroxin at 0, 10, 20 and 30 mL-1. Humic acid and nitroxin spray started in two true leaf stages for 3 times. The results showed that all humic acid and nitroxin treatments had significant effects on growth, yield and chemical characteristics including: Number of leaf/plant DM(%) plant, fruit diameter and length, fruit yield ha-1. Also, fruit quality such as fruit firmness, total soluble sugars, fruit skin total chlorophyll, fruit skin chlorophyll a, fruit skin chlorophyll b were affected by bio-fertilizers (humic acid and nitroxin). Spraying with humic acid 30 mL-1 can be a suitable treatment for enhancing growth and yield of cucumber plant.