Abstract: The hepatobiliary disease is frequently seen in companion animal practice. The present study was performed to investigate the protective effect of Yinzhihuang on liver injury in rabbits induced by CCl4. The results showed that compared with the liver injury model group, the dead rate obviously decreased, the serum ALT, AST and ALP activities were significantly decreased (p<0.01), the serum Total Bilirubin (TBIL) and Direct Bilirubin (DBIL) levels were significantly lower (p<0.05) and the degree of liver injury reduced significantly in Yinzhihuang protection groups. The data suggest that Yinzhihuang has obviously protective effect against liver injury induced by CCl4.