Authors : K. Rezai and T. Farboodnia
Abstract: Pea plants were grown in different concentrations of manganese (0, 25, 50, 100 and 200 ppm) during 15 days in culture solution. In this study, the effects of toxicity of manganese on chlorophyll content and antioxidant enzymes activity include catalase (CAT), ascorbate peroxidase (APX) and gauacol peroxidase (GPX) were investigated. The data showed that the low concentration of manganese (below 25 ppm) supplied in culture solution, induced decrease in chlorophyll content in pea plants and the high levels of manganese (above 50 ppm) inhibited chlorophyll synthesis in the leaves of this plants. Maximum and minimum rate of chlorophyll content were obtained in 25 and 200 ppm of manganese in culture solution, respectively. Our observations showed the close relationship between increase in manganese concentration and increase of antioxidant enzymes activity in pea plant grown in culture solution. The activity of APX in shoot and roots were higher than CAT and GPX. Data also suggest that the activity of antioxidant enzymes in shoot were higher than roots.
K. Rezai and T. Farboodnia , 2008. Manganese Toxicity Effects on Chlorophyll Content and Antioxidant Enzymes in Pea Plant (Pisum sativum L. c.v qazvin). Agricultural Journal, 3: 454-458.