Authors : Xun Wang, Haifeng Liu, Ling Zhao, Han Dong, Yuhao Wang, Di Sun, Yuyin Liu, Ruqi Yang and Xuewei Li
Abstract: To study the effects of Crude Polysaccharides from Catathelasma ventricosum (CVCP) on proliferation and differentiation of 3T3-L1 preadipocyte. 3T3-L1 cells were treated with culture media contained different concentrations of CVCP (0, 150, 300 and 600 μg mL-1) for 24 h and subsequently cell proliferation was assayed by MTT Method. The extent of the differentiation was determined by fluescence spectrophotometry and Nile Red staining on days 8 after treatment with culture media supplemented with different concentration CVCP (0, 150, 300 and 600 μg mL-1). As the results, the different concentration CVCP (150, 300 and 600 μg mL-1) promoted proliferation of 3T3-L1 preadipocytes when compared with the control (p<0.05). And it stimulated cell differentiation into adipocyte at low concentration (150 μg mL-1) while it inhibited cell differentiation into adipocyte at higher concentration (300 and 600 μg mL-1). But the difference was not significant (p>0.05). Besides, lipid content in 3T3-L1 cells treated with 150 μg mL-1 was significantly higher than treated with 600 μg mL-1 (p<0.05). It can be concluded from the results that the CVCP promoted proliferation of the 3T3-L1 cells 24 h after treatment. Meanwhile, it was involved to some extent in regulation of adipogenesis and lipogenesis.
Xun Wang, Haifeng Liu, Ling Zhao, Han Dong, Yuhao Wang, Di Sun, Yuyin Liu, Ruqi Yang and Xuewei Li, 2012. Effects of Crude Polysaccharides from Catathelasma ventricosum On the Proliferation and Differentiation of 3T3-L1 Cells. Agricultural Journal, 7: 187-190.