Authors : Rajib Sharke, Abdullah Al Faroque, Rafiqul Islam Sarder, Fazlul Awal Mollah and Shib Nath Pattadar
Abstract: The genetic variation of wild and hatchery populations of rohu individuals was analyzed using allozyme marker. The fish samples were collected from 3 main wilds (Halda, Jamuna and Padma) and 6 hatcheries of 3 origin (Brahmaputra and Rahim fish hatchery, Mymensingh; Rupali and Ma-fatema fish hatchery, Jessore; Bismillah and Rupali fish hatchery, Comilla) covering a wide geographical distribution of this species. The 6 enzymes (LDH, MDH, PGM, AAT, ADH GPI and G3PDH) encoded by 7 presumptive loci were screened and 4 (Mdh-1*, Gpi-1*, Gpi-2* and Pgm*) were found to be polymorphic which were interpretable in muscle with starch gel electrophoresis. The mean proportion of polymorphic loci, the mean proportion of heterozygous loci per individual, the average observed Heterozygosity (Ho) and expected Heterozygosity (He) were 42.85, 15.77, 0.125 and 0.184%, respectively for wild populations which were higher than the hatchery populations (33.33, 12.20, 0.075 and 0.094%, respectively). The highest gene flow value (26.725) and lowest population differentiation (0.009) found in the Halda and the Jamuna River populations indicate the close relationship among them Based on Nei (1972)’s UPGMA dendrogram, 9 populations of rohu were divided into 2 major clusters by the genetic distance of D = 0.092. The cluster-1 consisted of 3 wild populations and the cluster-2 consisted of 6 hatchery populations. The present study revealed that the considerable genetic variation is maintained among the wild and hatchery populations of rohu.
Rajib Sharke, Abdullah Al Faroque, Rafiqul Islam Sarder, Fazlul Awal Mollah and Shib Nath Pattadar, 2014. Genetic Variation of Wild and Hatchery Populations of Indian Major Carp, Rohu (Labeo rohita) in Bangladesh Using Allozyme Marker. Agricultural Journal, 9: 113-119.