Authors : Zhao Liansheng, Zheng Nan, Zhang Yangdong, Nan Xuemei, Hu Han, Cui Ruilian, Zhou Lingyun and Wang Jiaqi
Abstract: The study was conducted to evaluate the effect of Lipopolysaccharide (LPS) on milk fatty acid metabolism. The expression of genes for fatty acid metabolism in dairy cows was studied by incubating mammary epithelial cells with LPS at 0, 0.1 or 10 ng mL-1 for 24 h using RT-qRCR in vitro. LPS increased transporter CD36 mRNA expression (p<0.01). FABP3 mRNA expression (p>0.05) was not affected by LPS but the FABP4 mRNA expression was affected (p<0.05), decreasing 0.79 fold when LPS was provided at 10 ng mL-1. Treatment with 0.1 ng mL-1 LPS tend to increased fatty acid synthesis enzyme FASN, ACACA, ACSS2 and fatty acid metabolism gene PPARG, PPARGC1A mRNA expression (0.10>p>0.05) compared with the control. LPS alters fatty acid metabolism, functioning as an important factor affecting milk fat synthesis.
Zhao Liansheng, Zheng Nan, Zhang Yangdong, Nan Xuemei, Hu Han, Cui Ruilian, Zhou Lingyun and Wang Jiaqi, 2014. Lipopolysaccharide-Induced Alterations in mRNA Expression of Genes Related to Fatty Acid Metabolism. Agricultural Journal, 9: 99-102.