Abstract: Pathogenic Hog Cholera (HC) Virus (V) generally kills inoculated pigs before the development of detectable antibodies, making difficult to produce an immune sera against the ALD exposition pathogenic live-virus. With this last purpose a procedure was evaluated by inoculating 2 HCV seronegative pigs, initially with highly diluted dosages of challenge HCV (ALD strain/with an initial titer of 104.99CCID50/mL) and then progressively with more concentrated dosages (from 10-11 to 10-1). This allowed to produce the disease with a long incubation period. The 2 pigs presented fever and HC clinical signs and survived 30 and 38 days after inoculation, respectively. In a second phase, in an attempt to produce a HC immune serum, 5 groups of 4 susceptible pigs were immunized as follows Group (G) I, Negative Control; G II vaccinated with the PAV-250 HC vaccine; G III, inoculated with 1 mL ALD-HCV (104.0/mL); G IV, vaccinated and challenged with ALD-HCV; G V, vaccinated twice andALD-HCV challenged. G I was negative. HC-antibody titres of >=1:10 were detected at 14 Post Challenge (PC) days in Control Groups II, IV and V and at 15 PC. days in Group III. HC antibodies developed in G III because pigs had enough time for antibody production after being inoculated. The utilized challenge dilution (104.0 /mL) was satisfactory for obtaining immune sera against the pathogenic live HCV-ALD, in G III; Control groups II, IV and V also produced satisfactory immune sera.
Maria Antonia Coba Ayala , E. Pablo Correa Giron , Atalo Martinez Lara , Susana Mendoza Elvira , Abel Ciprian Carrasco , Oscar Torre and Edgar Aguilera Ceron , 2008. Antibody Development in Swine Against a Hog Cholera Lethal Strain. Journal of Animal and Veterinary Advances, 7: 94-99.