Authors : N.M. Budari, M.F. Ali, K.H. Ku Hamid, K.A. Khalil, S. Zainal and M. Musa
Abstract: This study was conducted to discover ultrasonic treatment as an innovative technology specifically for Escherichia coli wild type cell disruption for the disinfection process. Therefore, this study aims to evaluate the influence of process conditions through the performances of Flow Cell Ultrasound (FCUS) as the bacterial disruption. In order to determine the influence of the process condition through flow cell ultrasound, the research approach used in this study was measured by the disruption of Escherichia coli cells with inoculum (104 CFU/mL), sonication media Phosphate Buffer Saline (PBS) and peptone water, 35% amplitude and flow rate (70 mL/min). There was statistically a significance difference (p<0.05) between peptone water and PBS where PBS as the sonication media showed 6.93% higher cells disruption as compared to the peptone water. The percentage removal at 120 min treatment for cells disruption exhibited 98.55±2.51% these interpreted that the disruption of Escherichia coli wild type cells comply with first-order kinetics model and k = 0.0353 min-1. Furthermore, ANOVA elucidated that the temperature increases by time and p<0.05 and resulted in an average final temperature of 28.6°C which was within the non-lethal temperatures for Escherichia coli. Thus, the use of flow cell ultrasound was identified to be beneficial uses for water disinfection due to green process occurred during their removal performances.
N.M. Budari, M.F. Ali, K.H. Ku Hamid, K.A. Khalil, S. Zainal and M. Musa, 2017. Flow Cell Ultrasound Treatment: The Influence of Sonication Media and Temperature on the Disruption of Escherichia coli Wild Type Cells. Journal of Engineering and Applied Sciences, 12: 8751-8756.