Authors : Putut H. Riyadi, Yudhomenggolo S. Darmanto, Apri D. Anggo, Vivi E. Herawati and Retno A. Kurniasih
Abstract: This study aims to obtain a profile of the potential hydrolyzate of Portunus sp. with bioinformatics analysis. The material used in this study was Portunus sp. non-shell from the meat canning industry Portunus sp. Non-shell waste is hydrolyzed using papain enzyme to produce hydrolyzate. The hydrolyzate was screened with LC-HRMS and analyzed using swiss ADME, PASS and Pro Tox. LC-HRMS screening shows the results that there are twelve compounds, namely L-Phenylalanine, Tryptophan, trans-3-Indoleacrylic acid, Isoleucine, Betaine, Trigonelline, Adenosine, Tyrosine, Propionylcarnitine, Stachydrine, Maltose, Arginine. swiss ADME analysis shows that the hydrolyzate of Portunus sp. has potential as a pretty good drug ingredient based on the review of Lipinski’s rules and pharmacokinetic property. The results of the PASS analysis to predict the potential for biological activity indicate that the hydrolyzate of Portunus sp. Non-shell has potential as NADPH peroxidase inhibitors, Omptin inhibitors, Membrane integrity agonists, Preneoplastic conditions treatment, Phobic disorders treatment, Antiseborrheic, Pseudolysin inhibitors, Saccharopepsin inhibitors. Toxicity analysis based on Pro Tox predictions showed no potential Carcinogenicity, Immunotoxiticity, Mutagenicity. These results indicate that the hydrolyzate of Portunus sp. potentially as a nutraceutical and safe for consumption.
Putut H. Riyadi, Yudhomenggolo S. Darmanto, Apri D. Anggo, Vivi E. Herawati and Retno A. Kurniasih, 2020. Potential of Hydrolyzed Waste in Portunus sp. Non-Shell as Nutraceutical with Bioinformatics Analysis. Journal of Engineering and Applied Sciences, 15: 2327-2331.